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ProductsBack/SGCA / DAG2 Antibody

SGCA / DAG2 Antibody

LS-C746837
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSGCA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SGCA / DAG2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 29kDa/42kDa, while the observed MW by Western blot was 43kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 29kDa/42kDa, while the observed MW by Western blot was 43kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6442
  • Target name
    SGCA
  • Target description
    sarcoglycan alpha
  • Target synonyms
    50DAG, ADL, DAG2, DMDA2, LGMD2D, LGMDR3, SCARMD1, adhalin, alpha-sarcoglycan, 50 kDa dystrophin-associated glycoprotein, 50kD DAG, alpha-SG, dystroglycan-2, limb girdle muscular dystrophy 2D, sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein)
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SGCA using anti-SGCA antibody (PB9503). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug, Lane 2: Mouse Skeletal Muscle Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SGCA antigen affinity purified polyclonal antibody (Catalog # PB9503) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SGCA at approximately 43 kDa. The expected band size for SGCA is at 43 kDa.
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