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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Anti-SHOC2 Antibody

HPA048134
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSHOC2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SHOC2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8036
  • Target name
    SHOC2
  • Target description
    SHOC2 leucine rich repeat scaffold protein
  • Target synonyms
    NSLH1, SIAA0862, SOC2, SUR8, leucine-rich repeat protein SHOC-2, soc-2 suppressor of clear homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UQ13
  • Protein Name
    Leucine-rich repeat protein SHOC-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Western Blot Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate All lanes: SHOC2 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65, 60 kDa Observed band size: 65 kDa
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Figure 1. Western blot analysis of Shoc2/Sur8 using anti-Shoc2/Sur8 antibody (A07214-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human T-47D whole cell lysates, Lane 6: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Shoc2/Sur8 antigen affinity purified polyclonal antibody (Catalog # A07214-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Shoc2/Sur8 at approximately 70 kDa. The expected band size for Shoc2/Sur8 is at 70 kDa.
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Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Testis lysates; Lane 3: Mouse Esophagus lysates; Lane 4: Mouse Large intestine lysates; Lane 5: Mouse NIH/3T3 cell lysates; Lane 6: Rat Cerebrum lysates; Lane 7: Rat Testis lysates; Lane 8: Rat Esophagus lysates; Lane 9: Rat Large intestine lysates; Lane 10: Human A549 cell lysates; Lane 11: Human U937 cell lysates； Lane 12: Human HL-60 cell lysates probed with SHOC2 Polyclonal Antibody, Unconjugated (bs-7934R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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