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Western Blot Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate All lanes: SHOC2 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65, 60 kDa Observed band size: 65 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate All lanes: SHOC2 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65, 60 kDa Observed band size: 65 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate All lanes: SHOC2 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 65, 60 kDa Observed band size: 65 kDa

SHOC2 Antibody

CSB-PA891468LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSHOC2
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Overview

  • Supplier
    Cusabio
  • Product Name
    SHOC2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8036
  • Target name
    SHOC2
  • Target description
    SHOC2 leucine rich repeat scaffold protein
  • Target synonyms
    leucine-rich repeat protein SHOC-2; NSLH1; SIAA0862; SOC2; soc-2 suppressor of clear homolog; SUR8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UQ13
  • Protein Name
    Leucine-rich repeat protein SHOC-2
  • Scientific Description
    Regulatory subunit of protein phosphatase 1 (PP1c) that acts as a M-Ras/MRAS effector and participates in MAPK pathway activation. Upon M-Ras/MRAS activation, targets PP1c to specifically dephosphorylate the Ser-259 inhibitory site of RAF1 kinase and stimulate RAF1 activity at specialized signaling complexes.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Shoc2/Sur8 using anti-Shoc2/Sur8 antibody (A07214-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human T-47D whole cell lysates, Lane 6: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Shoc2/Sur8 antigen affinity purified polyclonal antibody (Catalog # A07214-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Shoc2/Sur8 at approximately 70 kDa. The expected band size for Shoc2/Sur8 is at 70 kDa.
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Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Testis lysates; Lane 3: Mouse Esophagus lysates; Lane 4: Mouse Large intestine lysates; Lane 5: Mouse NIH/3T3 cell lysates; Lane 6: Rat Cerebrum lysates; Lane 7: Rat Testis lysates; Lane 8: Rat Esophagus lysates; Lane 9: Rat Large intestine lysates; Lane 10: Human A549 cell lysates; Lane 11: Human U937 cell lysates； Lane 12: Human HL-60 cell lysates probed with SHOC2 Polyclonal Antibody, Unconjugated (bs-7934R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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