Figure 1. Western blot analysis of SHP2 using anti-SHP2 antibody (PB9675). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: Rat Cardiac Muscle Tissue Lysate at 50ug, Lane 4: Mouse Kidney Tissue Lysate at 50ug, Lane 5: HELA Whole Cell Lysate at 40ug, Lane 6: SW620 Whole Cell Lysate at 40ug, Lane 7: HEPG2 Whole Cell Lysate at 40ug, Lane 8: JURKAT Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHP2 antigen affinity purified polyclonal antibody (Catalog # PB9675) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHP2 at approximately 68 kDa. The expected band size for SHP2 is at 68 kDa.