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ProductsBack/Goat anti-SHP2 / PTPN11

Goat anti-SHP2 / PTPN11

EB05114
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetPTPN11
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-SHP2 / PTPN11 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HeLa cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5781
  • Target name
    PTPN11
  • Target description
    protein tyrosine phosphatase non-receptor type 11
  • Target synonyms
    BPTP3; CFC; JMML; METCDS; NS1; protein-tyrosine phosphatase 1D; protein-tyrosine phosphatase 2C; PTP-1D; PTP2C; SH2 domain-containing protein tyrosine phosphatase 2; SHP2; SH-PTP2; SH-PTP3; tyrosine-protein phosphatase non-receptor type 11
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_002825.3; NP_001317366.1. GeneIDs all Nonhuman: 19247 (mouse); 25622 (rat);. Purification: Antigen affinity purified. Names and symbols: PTPN11; SHP2; protein tyrosine phosphatase, non-receptor type 11 (Noonan syndrome 1); CFC; NS1; BPTP3; PTP2C; SHP-2; PTP-1D; SH-PTP2; SH-PTP3; Noonan syndrome 1; protein-tyrosine phosphatase 2C; protein tyrosine phosphatase, non-receptor type 11; MGC14433
  • Reactivity Supplier
    Human, Mouse, Rat, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SHP2 using anti-SHP2 antibody (PB9675). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: Rat Cardiac Muscle Tissue Lysate at 50ug, Lane 4: Mouse Kidney Tissue Lysate at 50ug, Lane 5: HELA Whole Cell Lysate at 40ug, Lane 6: SW620 Whole Cell Lysate at 40ug, Lane 7: HEPG2 Whole Cell Lysate at 40ug, Lane 8: JURKAT Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHP2 antigen affinity purified polyclonal antibody (Catalog # PB9675) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHP2 at approximately 68 kDa. The expected band size for SHP2 is at 68 kDa.
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