More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-SIRT5 [RAB-C179]

Anti-SIRT5 [RAB-C179]

Ab01870-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation
Product group Antibodies
TargetSIRT5
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-SIRT5 [RAB-C179]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody has been used and recommended for the following methods: FC; spiked-IP; IP-MS; IF.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation
  • Applications Supplier
    FC; spiked-IP; IP-MS; IF
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RAB-C179
  • Gene ID23408
  • Target name
    SIRT5
  • Target description
    sirtuin 5
  • Target synonyms
    NAD-dependent deacetylase sirtuin-5; NAD-dependent lysine demalonylase and desuccinylase sirtuin-5, mitochondrial; NAD-dependent protein deacylase sirtuin-5, mitochondrial; silent mating type information regulation 2, S.cerevisiae, homolog 5; SIR2L5; sir2-like 5; sirtuin type 5
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9NXA8
  • Protein Name
    NAD-dependent protein deacylase sirtuin-5, mitochondrial
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was obtained by recombinant antibody (rAb) phage display recognizing SIRT5 protein under non-denaturing conditions.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Figure 1. Western blot analysis of SIRT5 using anti-SIRT5 antibody (A02395-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT5 antigen affinity purified polyclonal antibody (Catalog # A02395-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT5 at approximately 34 kDa. The expected band size for SIRT5 is at 34 kDa.
Product group Antibodies
Boster Bio
Anti-SIRT5 Antibody Picoband(r)A02395-3-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetSIRT5
  • SizePrice
Product group Antibodies
Assay Genie
[KO Validated] SIRT5 AntibodyCAB5784
TargetSIRT5
  • SizePrice
Mouse splenocytes probed with Rabbit Anti-SIRT5 Polyclonal Antibody, Unconjugated (bs-9456R) (green) at 1:50 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
Product group Antibodies
Bioss Antibodies
SIRT5 Polyclonal AntibodyBS-9456R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetSIRT5
  • SizePrice
Product group Antibodies
Biomatik
Sirtuin 5 (SIRT5) Polyclonal AntibodyCAU22619
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetSIRT5
  • SizePrice
Anti-SIRT5 Antibody
Product group Antibodies
Antibodies.com
Anti-SIRT5 AntibodyA306711
ApplicationsWestern Blot
  • SizePrice
Western blot analysis using anti-Sirtuin 5 (human), pAb (Prod. No. AG-25A-0100) at 1:2000 dilution. 1: Human Sirtuin 5 (intact form) (His-tagged). 2: Human Sirtuin 1 (His-tagged). 3: Human Sirtuin 2 (His-tagged). 4: Human Sirtuin
Product group Antibodies
AdipoGen Life Sciences
anti-Sirtuin 5 (human), pAbAG-25A-0100
ApplicationsWestern Blot, ELISA
TargetSIRT5
  • SizePrice
Product group Antibodies
RayBiotech
Anti-SIRT5 Antibody144-65992
ApplicationsWestern Blot
TargetSIRT5
  • SizePrice
Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Product group Antibodies
Atlas Antibodies
Anti-SIRT5 AntibodyHPA022002
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetSIRT5
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
SIRT5 / Sirtuin 5 AntibodyLS-C405797
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetSIRT5
  • SizePrice
Product group Antibodies
GeneTex
SIRT5 antibodyGTX117824
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetSIRT5
  • SizePrice