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Western blot analysis using anti-Sirtuin 5 (human), pAb (Prod. No. AG-25A-0100) at 1:2000 dilution. 1: Human Sirtuin 5 (intact form) (His-tagged). 2: Human Sirtuin 1 (His-tagged). 3: Human Sirtuin 2 (His-tagged). 4: Human Sirtuin
Western blot analysis using anti-Sirtuin 5 (human), pAb (Prod. No. AG-25A-0100) at 1:2000 dilution. 1: Human Sirtuin 5 (intact form) (His-tagged). 2: Human Sirtuin 1 (His-tagged). 3: Human Sirtuin 2 (His-tagged). 4: Human Sirtuin
Western blot analysis using anti-Sirtuin 5 (human), pAb (Prod. No. AG-25A-0100) at 1:2000 dilution. 1: Human Sirtuin 5 (intact form) (His-tagged). 2: Human Sirtuin 1 (His-tagged). 3: Human Sirtuin 2 (His-tagged). 4: Human Sirtuin

anti-Sirtuin 5 (human), pAb

AG-25A-0100
AdipoGen Life Sciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSIRT5
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Overview

  • Supplier
    AdipoGen Life Sciences
  • Product Name
    anti-Sirtuin 5 (human), pAb
  • Delivery Days Customer
    10
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID23408
  • Target name
    SIRT5
  • Target description
    sirtuin 5
  • Target synonyms
    NAD-dependent deacetylase sirtuin-5; NAD-dependent lysine demalonylase and desuccinylase sirtuin-5, mitochondrial; NAD-dependent protein deacylase sirtuin-5, mitochondrial; silent mating type information regulation 2, S.cerevisiae, homolog 5; SIR2L5; sir2-like 5; sirtuin type 5
  • Host
    Rabbit
  • Protein IDQ9NXA8
  • Protein Name
    NAD-dependent protein deacylase sirtuin-5, mitochondrial
  • Scientific Description
    Polyclonal Antibody. Recognizes human sirtuin 5 (intact form). Detects a band of ~37kDa by Western blot. Source: Rabbit. Applications: ELISA, WB. Liquid. 0.2microm-filtered solution in PBS, pH 7.4. Contains no preservatives. Sirtuin 5 is a NAD-dependent protein deacetylase that activates CPS1 and contributes to the regulation of blood ammonia levels during fasting. Can deacetylate cytochrome c and a number of other proteins (in vitro). - Sirtuin 5 is a NAD-dependent protein deacetylase that activates CPS1 and contributes to the regulation of blood ammonia levels during fasting. Can deacetylate cytochrome c and a number of other proteins (in vitro).
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SIRT5 using anti-SIRT5 antibody (A02395-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT5 antigen affinity purified polyclonal antibody (Catalog # A02395-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT5 at approximately 34 kDa. The expected band size for SIRT5 is at 34 kDa.
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