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Immunofluorescent staining of human cell line RT4 shows localization to plasma membrane.
Immunofluorescent staining of human cell line RT4 shows localization to plasma membrane.
Immunofluorescent staining of human cell line RT4 shows localization to plasma membrane.

Anti-SLC14A1 Antibody

HPA077233
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSLC14A1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SLC14A1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6563
  • Target name
    SLC14A1
  • Target description
    solute carrier family 14 member 1 (Kidd blood group)
  • Target synonyms
    HsT1341; HUT11; JK; Jk(b); Kidd blood group antigen; RACH1; RACH2; SLC14A1 JK; solute carrier family 14 (urea transporter), member 1 (Kidd blood group); urea transporter 1; urea transporter JK glycoprotein; urea transporter, erythrocyte; urea transporter-B1; UT1; UT-B1; UTE
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13336
  • Protein Name
    Urea transporter 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of UT-B/SLC14A1 using anti-UT-B/SLC14A1 antibody (A04926-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UT-B/SLC14A1 antigen affinity purified polyclonal antibody (Catalog # A04926-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UT-B/SLC14A1 at approximately 65 kDa. The expected band size for UT-B/SLC14A1 is at 43 kDa.
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