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Urea Transporter, Erythrocyte (UTE) Monoclonal Antibody

CAU34400
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityPorcine
TargetSLC14A1
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Overview

  • Supplier
    Biomatik
  • Product Name
    Urea Transporter, Erythrocyte (UTE) Monoclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    C4
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6563
  • Target name
    SLC14A1
  • Target description
    solute carrier family 14 member 1 (Kidd blood group)
  • Target synonyms
    HUT11, HUT11A, HsT1341, JK, Jk(a), Jk(b), RACH1, RACH2, UT-B1, UT1, UTE, urea transporter 1, Kidd blood group antigen, SLC14A1 JK, mutant KIDD blood group antigen, solute carrier family 14 (urea transporter), member 1 (Kidd blood group), urea transporter JK glycoprotein, urea transporter, erythrocyte, urea transporter-B1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ13336
  • Protein Name
    Urea transporter 1
  • Scientific Description
    The Urea Transporter, Erythrocyte (UTE) Monoclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Porcine
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of UT-B/SLC14A1 using anti-UT-B/SLC14A1 antibody (A04926-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UT-B/SLC14A1 antigen affinity purified polyclonal antibody (Catalog # A04926-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UT-B/SLC14A1 at approximately 65 kDa. The expected band size for UT-B/SLC14A1 is at 43 kDa.
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