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Immunohistochemical staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.

Anti-SLC22A7 Antibody

HPA030220
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSLC22A7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SLC22A7
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10864
  • Target name
    SLC22A7
  • Target description
    solute carrier family 22 member 7
  • Target synonyms
    hOAT11; hOAT2; liver-specific transporter; NLT; novel liver transporter; OAT2; organic anion transporter 11; organic anion transporter 2; solute carrier family 22 (organic anion transporter), member 7; solute carrier family 22 member 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y694
  • Protein Name
    Solute carrier family 22 member 7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OAT2/SLC22A7 using anti-OAT2/SLC22A7 antibody (A05273-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OAT2/SLC22A7 antigen affinity purified polyclonal antibody (Catalog # A05273-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OAT2/SLC22A7 at approximately 60 kDa. The expected band size for OAT2/SLC22A7 is at 60 kDa.
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