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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Anti-SLC27A4 Antibody

HPA007293
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetSLC27A4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SLC27A4
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10999
  • Target name
    SLC27A4
  • Target description
    solute carrier family 27 member 4
  • Target synonyms
    ACSVL4; arachidonate--CoA ligase; FATP4; IPS; long-chain fatty acid transport protein 4; long-chain-fatty-acid--CoA ligase; solute carrier family 27 (fatty acid transporter), member 4; very long-chain acyl-CoA synthetase 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6P1M0
  • Protein Name
    Long-chain fatty acid transport protein 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FATP4/SLC27A4 using anti-FATP4/SLC27A4 antibody (A05299-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FATP4/SLC27A4 antigen affinity purified polyclonal antibody (Catalog # A05299-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FATP4/SLC27A4 at approximately 70 kDa. The expected band size for FATP4/SLC27A4 is at 72 kDa.
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