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FATP4 Recombinant Antibody

BSM-62978R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetSLC27A4
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    FATP4 Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID10999
  • Target name
    SLC27A4
  • Target description
    solute carrier family 27 member 4
  • Target synonyms
    ACSVL4; arachidonate--CoA ligase; FATP4; IPS; long-chain fatty acid transport protein 4; long-chain-fatty-acid--CoA ligase; solute carrier family 27 (fatty acid transporter), member 4; very long-chain acyl-CoA synthetase 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6P1M0
  • Protein Name
    Long-chain fatty acid transport protein 4
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FATP4/SLC27A4 using anti-FATP4/SLC27A4 antibody (A05299-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FATP4/SLC27A4 antigen affinity purified polyclonal antibody (Catalog # A05299-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FATP4/SLC27A4 at approximately 70 kDa. The expected band size for FATP4/SLC27A4 is at 72 kDa.
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