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Immunohistochemical staining of human spleen shows strong membranous positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows strong membranous positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows strong membranous positivity in cells in red pulp.

Anti-SLC40A1 Antibody

HPA065634
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSLC40A1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SLC40A1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID30061
  • Target name
    SLC40A1
  • Target description
    solute carrier family 40 member 1
  • Target synonyms
    FPN1; HFE4; IREG1; iron regulated gene 1; MST079; MSTP079; MTP1; SLC11A3; solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3; solute carrier family 40 (iron-regulated transporter), member 1; solute carrier family 40 member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NP59
  • Protein Name
    Solute carrier family 40 member 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SLC40A1 using anti-SLC40A1 antibody (A01953-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC40A1 antigen affinity purified polyclonal antibody (Catalog # A01953-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC40A1 at approximately 70 kDa. The expected band size for SLC40A1 is at 63 kDa.
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