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Anti-Smad1 Antibody

130-00004
RayBiotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSMAD1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-Smad1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID4086
  • Target name
    SMAD1
  • Target description
    SMAD family member 1
  • Target synonyms
    BSP1; BSP-1; JV41; JV4-1; MAD, mothers against decapentaplegic homolog 1; MADH1; MADR1; Mad-related protein 1; mothers against decapentaplegic homolog 1; mothers against DPP homolog 1; SMAD, mothers against DPP homolog 1; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1
  • Host
    Rabbit
  • Protein IDQ15797
  • Protein Name
    Mothers against decapentaplegic homolog 1
  • Scientific Description
    Rabbit Anti-Smad1 Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Smad1 + Smad5 Antibody(bs-1619R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Product group Antibodies
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Figure 1. Western blot analysis of SMAD1 using anti-SMAD1 antibody (A00728-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate, Lane 2: rat testis tissue lysate, Lane 3: mouse brain tissue lysate, Lane 4: mouse kidney tissue lysate, Lane 5: mouse testis tissue lysate, Lane 6: human Hela cell lysate, Lane 7: human A375 cell lysate, Lane 8: human HepG2 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD1 antigen affinity purified polyclonal antibody (Catalog # A00728-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD1 at approximately 52,60KD. The expected band size for SMAD1 is at 48, 52, 60KD.
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