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Anti-Smad2 Antibody

Anti-Smad2 Antibody

A40174
Antibodies.com
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-Smad2 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Mouse monoclonal antibody to Smad2
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SMAD2 using anti-SMAD2 antibody (A00090-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates Lane 2: rat liver tissue lysates Lane 3: mouse testicular tissue lysates Lane 4: mouse heart tissue lysates Lane 5: mouse lung tissue lysates Lane 6: mouse lung tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 antigen affinity purified polyclonal antibody (Catalog # A00090-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2 at approximately 60KD. The expected band size for SMAD2 is at 52KD.
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