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Immunohistochemical staining of human small intestine shows strong nuclear immunoreactivity in glandular cells and in connective tissue cells.
Immunohistochemical staining of human small intestine shows strong nuclear immunoreactivity in glandular cells and in connective tissue cells.
Immunohistochemical staining of human small intestine shows strong nuclear immunoreactivity in glandular cells and in connective tissue cells.

Anti-SMCHD1 Antibody

AMAB91282
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSMCHD1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SMCHD1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL4270
  • Conjugate
    Unconjugated
  • Gene ID23347
  • Target name
    SMCHD1
  • Target description
    structural maintenance of chromosomes flexible hinge domain containing 1
  • Target synonyms
    BAMS; FSHD2; SMC hinge domain-containing protein 1; structural maintenance of chromosomes flexible hinge domain-containing protein 1
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDA6NHR9
  • Protein Name
    Structural maintenance of chromosomes flexible hinge domain-containing protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of SMCHD1 using anti-SMCHD1 antibody (A04751-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMCHD1 antigen affinity purified polyclonal antibody (Catalog # A04751-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMCHD1 at approximately 226 kDa. The expected band size for SMCHD1 is at 226 kDa.
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