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Anti-SMURF1 [RAB-C449]

Ab01883-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence
Product group Antibodies
TargetSMURF1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-SMURF1 [RAB-C449]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody has been used and recommended for the following methods: FC; spiked-IP; IF.
  • Applications
    Flow Cytometry, ImmunoFluorescence
  • Applications Supplier
    FC; spiked-IP; IF
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RAB-C449
  • Gene ID57154
  • Target name
    SMURF1
  • Target description
    SMAD specific E3 ubiquitin protein ligase 1
  • Target synonyms
    E3 ubiquitin-protein ligase SMURF1, E3 ubiquitin ligase SMURF1, HECT-type E3 ubiquitin transferase SMURF1, Smad ubiquitination regulatory factor 1, Smad-specific E3 ubiquitin ligase 1, hSMURF1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9HCE7
  • Protein Name
    E3 ubiquitin-protein ligase SMURF1
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was obtained by recombinant antibody (rAb) phage display recognizing SMURF1 protein under non-denaturing conditions.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SMURF1 using anti-SMURF1 antibody (PB9892). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMURF1 antigen affinity purified polyclonal antibody (Catalog # PB9892) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMURF1 at approximately 86 kDa. The expected band size for SMURF1 is at 86 kDa.
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