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Immunohistochemical staining of human placenta shows strong membranous positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong membranous positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong membranous positivity in trophoblastic cells.

Anti-SNAP23 Antibody

HPA001214
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSNAP23
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SNAP23
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8773
  • Target name
    SNAP23
  • Target description
    synaptosome associated protein 23
  • Target synonyms
    HsT17016, SNAP-23, SNAP23A, SNAP23B, synaptosomal-associated protein 23, synaptosomal-associated protein, 23kD, synaptosomal-associated protein, 23kDa, synaptosome associated protein 23kDa, vesicle-membrane fusion protein SNAP-23
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00161
  • Protein Name
    Synaptosomal-associated protein 23
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SNAP23 using anti-SNAP23 antibody (A02487-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAP23 antigen affinity purified polyclonal antibody (Catalog # A02487-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAP23 at approximately 25 kDa. The expected band size for SNAP23 is at 23 kDa.
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