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Western Blot analysis of HepG2 cells using SNAP 23 Polyclonal Antibody
Western Blot analysis of HepG2 cells using SNAP 23 Polyclonal Antibody
Western Blot analysis of HepG2 cells using SNAP 23 Polyclonal Antibody

SNAP23 Antibody

CSB-PA005176
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSNAP23
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Overview

  • Supplier
    Cusabio
  • Product Name
    SNAP23 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8773
  • Target name
    SNAP23
  • Target description
    synaptosome associated protein 23
  • Target synonyms
    HsT17016; SNAP-23; SNAP23A; SNAP23B; synaptosomal-associated protein 23; synaptosomal-associated protein, 23kD; synaptosomal-associated protein, 23kDa; synaptosome associated protein 23kDa; vesicle-membrane fusion protein SNAP-23
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00161
  • Protein Name
    Synaptosomal-associated protein 23
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SNAP23 using anti-SNAP23 antibody (A02487-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNAP23 antigen affinity purified polyclonal antibody (Catalog # A02487-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNAP23 at approximately 25 kDa. The expected band size for SNAP23 is at 23 kDa.
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Lane 1: Hela Cells; Probed with SNAP23 (1G3) Monoclonal Antibody (bsm-54062R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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