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Immunohistochemical staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic and nuclear positivity in glandular cells.

Anti-SNRNP40 Antibody

HPA027345
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSNRNP40
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SNRNP40 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9410
  • Target name
    SNRNP40
  • Target description
    small nuclear ribonucleoprotein U5 subunit 40
  • Target synonyms
    40K, HPRP8BP, PRP8BP, PRPF8BP, SPF38, WDR57, U5 small nuclear ribonucleoprotein 40 kDa protein, 38 kDa-splicing factor, Prp8-binding protein, U5 snRNP 40 kDa protein, U5 snRNP-specific 40 kDa protein, U5-40K, U5-40kD protein, WD repeat domain 57 (U5 snRNP specific), WD repeat-containing protein 57, small nuclear ribonucleoprotein 40kDa (U5), small nuclear ribonucleoprotein, U5 40kDa subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96DI7
  • Protein Name
    U5 small nuclear ribonucleoprotein 40 kDa protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of SNRNP40 using anti-SNRNP40 antibody (A12991-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRNP40 antigen affinity purified polyclonal antibody (Catalog # A12991-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNRNP40 at approximately 37 kDa. The expected band size for SNRNP40 is at 39 kDa.
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