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Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.

Anti-SNRPA Antibody

HPA046440
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSNRPA
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SNRPA
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6626
  • Target name
    SNRPA
  • Target description
    small nuclear ribonucleoprotein polypeptide A
  • Target synonyms
    Mud1; U1 small nuclear ribonucleoprotein A; U1 small nuclear RNP-specific A; U1 snRNP A; U1 snRNP-specific protein A; U1A; U1-A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09012
  • Protein Name
    U1 small nuclear ribonucleoprotein A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-U1A/SNRPA antigen affinity purified polyclonal antibody (Catalog # A08780-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for U1A/SNRPA at approximately 31 kDa. The expected band size for U1A/SNRPA is at 31 kDa.
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