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ProductsBack/Anti-U1A [U11B3]

Anti-U1A [U11B3]

Ab03412-23.0
Absolute Antibody
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSNRPA
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-U1A [U11B3]
  • Delivery Days Customer
    7
  • Application Supplier Note
    The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected U1A by western blot analysis (Hof et al., 2008; PMID: 18062792).
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA; WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    U11B3
  • Gene ID6626
  • Target name
    SNRPA
  • Target description
    small nuclear ribonucleoprotein polypeptide A
  • Target synonyms
    Mud1; U1 small nuclear ribonucleoprotein A; U1 small nuclear RNP-specific A; U1 snRNP A; U1 snRNP-specific protein A; U1A; U1-A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09012
  • Protein Name
    U1 small nuclear ribonucleoprotein A
  • Scientific Description
    This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Chicken scFv format for improved compatibility with existing reagents assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The original antibody was generated by immunizing a barnevelder chicken with an antibody antigen cocktail comprising recombinant purified human antigens annexin XI, CENP-B, Topo, Jo-1, Ro60, Rpp38 and U1A in complete Freund's adjuvant. B cells that were harvested from the bone marrow and peripheral blood lymphocytes of immunized chickens was used to generate recombinant scFv phage display libraries. The specific clone was identified by panning the library against specific antigen.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-U1A/SNRPA antigen affinity purified polyclonal antibody (Catalog # A08780-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for U1A/SNRPA at approximately 31 kDa. The expected band size for U1A/SNRPA is at 31 kDa.
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