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Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.

Anti-SNRPD2 Antibody

HPA041437
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSNRPD2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SNRPD2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6633
  • Target name
    SNRPD2
  • Target description
    small nuclear ribonucleoprotein D2 polypeptide
  • Target synonyms
    SMD2, SNRPD1, Sm-D2, small nuclear ribonucleoprotein Sm D2, small nuclear ribonucleoprotein D2 polypeptide 16.5kDa, snRNP core protein D2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62316
  • Protein Name
    Small nuclear ribonucleoprotein Sm D2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SNRPD2 using anti-SNRPD2 antibody (A12026-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRPD2 antigen affinity purified polyclonal antibody (Catalog # A12026-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNRPD2 at approximately 16 kDa. The expected band size for SNRPD2 is at 14 kDa.
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