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Immunohistochemical staining of human breast shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human breast shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human breast shows moderate cytoplasmic positivity in glandular cells.

Anti-SNX1 Antibody

HPA047373
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSNX1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SNX1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6642
  • Target name
    SNX1
  • Target description
    sorting nexin 1
  • Target synonyms
    HsT17379; sorting nexin 1A; sorting nexin-1; VPS5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13596
  • Protein Name
    Sorting nexin-1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SNX1 using anti-SNX1 antibody (A02692-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX1 antigen affinity purified polyclonal antibody (Catalog # A02692-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNX1 at approximately 70-75 kDa. The expected band size for SNX1 is at 59 kDa.
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Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
Product group Antibodies
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Anti-SNX1 AntibodyHPA052761
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TargetSNX1
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Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
Product group Antibodies
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Anti-SNX1 AntibodyHPA052761
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TargetSNX1
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