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Immunohistochemical staining of human endometrium shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human endometrium shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human endometrium shows cytoplasmic positivity in glandular cells.

Anti-SOCS5 Antibody

HPA020884
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSOCS5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SOCS5
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9655
  • Target name
    SOCS5
  • Target description
    suppressor of cytokine signaling 5
  • Target synonyms
    CIS6; CIS-6; Cish5; CISH6; cytokine-inducible SH2 protein 6; cytokine-inducible SH2-containing protein 5; SOCS-5; suppressor of cytokine signaling 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75159
  • Protein Name
    Suppressor of cytokine signaling 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SOCS5 using anti-SOCS5 antibody (A05989-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS5 antigen affinity purified polyclonal antibody (Catalog # A05989-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOCS5 at approximately 61KD. The expected band size for SOCS5 is at 61KD.
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