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WB analysis of Molt-4 cell lysate using GTX89655 SOCS5 antibody, N-term. Dilution : 0.3microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of Molt-4 cell lysate using GTX89655 SOCS5 antibody, N-term. Dilution : 0.3microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of Molt-4 cell lysate using GTX89655 SOCS5 antibody, N-term. Dilution : 0.3microg/ml Loading : 35microg protein in RIPA buffer

SOCS5 antibody, N-term

GTX89655
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetSOCS5
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Overview

  • Supplier
    GeneTex
  • Product Name
    SOCS5 antibody, N-term
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 0.3-1microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9655
  • Target name
    SOCS5
  • Target description
    suppressor of cytokine signaling 5
  • Target synonyms
    CIS6; CIS-6; Cish5; CISH6; cytokine-inducible SH2 protein 6; cytokine-inducible SH2-containing protein 5; SOCS-5; suppressor of cytokine signaling 5
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDO75159
  • Protein Name
    Suppressor of cytokine signaling 5
  • Scientific Description
    The protein encoded by this gene contains a SH2 domain and a SOCS BOX domain. The protein thus belongs to the suppressor of cytokine signaling (SOCS) family, also known as STAT-induced STAT inhibitor (SSI) protein family. SOCS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The specific function of this protein has not yet been determined. Two alternatively spliced transcript variants encoding an identical protein have been reported. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SOCS5 using anti-SOCS5 antibody (A05989-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS5 antigen affinity purified polyclonal antibody (Catalog # A05989-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOCS5 at approximately 61KD. The expected band size for SOCS5 is at 61KD.
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