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Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neurons and glial cells.
Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neurons and glial cells.
Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neurons and glial cells.

Anti-SOX4 Antibody

HPA029901
Atlas Antibodies
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSOX4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SOX4
  • Delivery Days Customer
    4
  • Applications
    ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6659
  • Target name
    SOX4
  • Target description
    SRY-box transcription factor 4
  • Target synonyms
    CSS10, EVI16, IDDSDF, transcription factor SOX-4, SRY (sex determining region Y)-box 4, SRY-box 4, SRY-related HMG-box gene 4, ecotropic viral integration site 16
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ06945
  • Protein Name
    Transcription factor SOX-4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of SOX4 using anti-SOX4 antibody (PB9618). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 Whole Cell Lysate, Lane 2: 293T Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX4 antigen affinity purified polyclonal antibody (Catalog # PB9618) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX4 at approximately 47KD. The expected band size for SOX4 is at 47KD.
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