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Anti-SOX5 Antibody

144-06985
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSOX5
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SOX5 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6660
  • Target name
    SOX5
  • Target description
    SRY-box transcription factor 5
  • Target synonyms
    LAMSHF; L-SOX5; L-SOX5B; L-SOX5F; SRY (sex determining region Y)-box 5; SRY-box 5; transcription factor SOX-5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35711
  • Protein Name
    Transcription factor SOX-5
  • Scientific Description
    SOX5 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SOX5 using anti-SOX5 antibody (PB9507). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Rat Testis Tissue Lysate at 50ug, Lane 3: Rat Brain Tissue Lysate at 50ug, Lane 4: HELA Whole Cell Lysate at 40ug, Lane 5: A549 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX5 antigen affinity purified polyclonal antibody (Catalog # PB9507) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX5 at approximately 84 kDa. The expected band size for SOX5 is at 84 kDa.
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