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ProductsBack/SOX5 Antibody (aa1-120)

SOX5 Antibody (aa1-120)

LS-C346170
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSOX5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SOX5 Antibody (aa1-120)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 41kDa/71kDa/82kDa/84kDa, while the observed MW by Western blot was 70kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:50 - 1:100), IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 41kDa/71kDa/82kDa/84kDa, while the observed MW by Western blot was 70kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6660
  • Target name
    SOX5
  • Target description
    SRY-box transcription factor 5
  • Target synonyms
    L-SOX5, L-SOX5B, L-SOX5F, LAMSHF, transcription factor SOX-5, SRY (sex determining region Y)-box 5, SRY-box 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SOX5 using anti-SOX5 antibody (PB9507). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Rat Testis Tissue Lysate at 50ug, Lane 3: Rat Brain Tissue Lysate at 50ug, Lane 4: HELA Whole Cell Lysate at 40ug, Lane 5: A549 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX5 antigen affinity purified polyclonal antibody (Catalog # PB9507) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX5 at approximately 84 kDa. The expected band size for SOX5 is at 84 kDa.
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