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Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.

Anti-SP3 Antibody

HPA032146
Atlas Antibodies
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSP3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SP3 Antibody
  • Delivery Days Customer
    4
  • Applications
    ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6670
  • Target name
    SP3
  • Target description
    Sp3 transcription factor
  • Target synonyms
    SPR2, transcription factor Sp3, GC-binding transcription factor Sp3, specificity protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02447
  • Protein Name
    Transcription factor Sp3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of SP3 using anti-SP3 antibody (PB9508). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: PC-12 Whole Cell Lysate at 40ug, Lane 2: HEPA Whole Cell Lysate at 40ug, Lane 3: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP3 antigen affinity purified polyclonal antibody (Catalog # PB9508) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SP3 at approximately 82 kDa. The expected band size for SP3 is at 82 kDa.
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