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SP3 Antibody

CAB12790
Assay Genie
TargetSP3
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    SP3 Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID6670
  • Target name
    SP3
  • Target description
    Sp3 transcription factor
  • Target synonyms
    SPR2, transcription factor Sp3, GC-binding transcription factor Sp3, specificity protein 3
  • Host
    Rabbit
  • Protein IDQ02447
  • Protein Name
    Transcription factor Sp3
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of SP3 using anti-SP3 antibody (PB9508). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: PC-12 Whole Cell Lysate at 40ug, Lane 2: HEPA Whole Cell Lysate at 40ug, Lane 3: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP3 antigen affinity purified polyclonal antibody (Catalog # PB9508) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SP3 at approximately 82 kDa. The expected band size for SP3 is at 82 kDa.
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