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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in in cells in seminiferous ducts.

Anti-SPAG5 Antibody

HPA022008
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSPAG5
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SPAG5
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10615
  • Target name
    SPAG5
  • Target description
    sperm associated antigen 5
  • Target synonyms
    astrin; DEEPEST; hMAP126; MAP126; mitotic spindle associated protein p126; mitotic spindle coiled-coil related protein; sperm-associated antigen 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96R06
  • Protein Name
    Sperm-associated antigen 5
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Astrin/Deepest/SPAG5 using anti-Astrin/Deepest/SPAG5 antibody (A07062-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Astrin/Deepest/SPAG5 antigen affinity purified polyclonal antibody (Catalog # A07062-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Astrin/Deepest/SPAG5 at approximately 130KD, 150KD. The expected band size for Astrin/Deepest/SPAG5 is at 130KD, 150KD.
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