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The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA440250(SPAG5 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA440250(SPAG5 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA440250(SPAG5 Antibody) at dilution 1/35, on the right is treated with fusion protein. (Original magnification: x200)

SPAG5 Antibody

CSB-PA440250
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSPAG5
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Overview

  • Supplier
    Cusabio
  • Product Name
    SPAG5 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10615
  • Target name
    SPAG5
  • Target description
    sperm associated antigen 5
  • Target synonyms
    DEEPEST, MAP126, hMAP126, sperm-associated antigen 5, astrin, mitotic spindle associated protein p126, mitotic spindle coiled-coil related protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96R06
  • Protein Name
    Sperm-associated antigen 5
  • Scientific Description
    Sperm-associated antigen 5 is a protein that in humans is encoded by the SPAG5 gene. This gene encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles. Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. The astrin (SPAG5)-kinastrin (SKAP) complex promotes stable microtubule-kinetochore attachments.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Astrin/Deepest/SPAG5 using anti-Astrin/Deepest/SPAG5 antibody (A07062-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Astrin/Deepest/SPAG5 antigen affinity purified polyclonal antibody (Catalog # A07062-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Astrin/Deepest/SPAG5 at approximately 130KD, 150KD. The expected band size for Astrin/Deepest/SPAG5 is at 130KD, 150KD.
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