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Immunohistochemical staining of human testis shows moderate cytopalsmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate cytopalsmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate cytopalsmic positivity in cells in seminiferous ducts.

Anti-SPATA5 Antibody

HPA036451
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAFG2A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SPATA5 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID166378
  • Target name
    AFG2A
  • Target description
    AAA ATPase AFG2A
  • Target synonyms
    AFG2, EHLMRS, NEDHSB, SPAF, SPATA5, ATPase family gene 2 protein homolog A, AFG2 AAA ATPase homolog A, ATPase family gene 2 homolog, ATPase family protein 2 homolog, ribosome biogenesis protein SPATA5, spermatogenesis associated 5, spermatogenesis associated factor SPAF, spermatogenesis-associated factor protein, spermatogenesis-associated protein 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NB90
  • Protein Name
    ATPase family gene 2 protein homolog A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SPATA5 using anti-SPATA5 antibody (A06364-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse testis tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPATA5 antigen affinity purified polyclonal antibody (Catalog # A06364-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPATA5 at approximately 98 kDa. The expected band size for SPATA5 is at 98 kDa.
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