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Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in subset of renal tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in subset of renal tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in subset of renal tubules.

Anti-SPECC1L Antibody

HPA070614
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetSPECC1L
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SPECC1L
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23384
  • Target name
    SPECC1L
  • Target description
    sperm antigen with calponin homology and coiled-coil domains 1 like
  • Target synonyms
    cytokinesis and spindle organization A; cytospin-A; CYTSA; GBBB2; OBLFC1; renal carcinoma antigen NY-REN-22; SPECC1-like protein; TBHS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ69YQ0
  • Protein Name
    Cytospin-A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SPECC1L using anti-SPECC1L antibody (A09120-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPECC1L antigen affinity purified polyclonal antibody (Catalog # A09120-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPECC1L at approximately 150 kDa. The expected band size for SPECC1L is at 125 kDa.
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Lane 1: HL60 Cells; Lane 2: NIH/3T3 Cells; Lane 3: U2OS Cells; Lane 4: HL60 Cells; Probed with SPECC1L Monoclonal Antibody (bs-7773R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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