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ProductsBack/SPECC1L Polyclonal Antibody
Lane 1: HL60 Cells; Lane 2: NIH/3T3 Cells; Lane 3: U2OS Cells; Lane 4: HL60 Cells; Probed with SPECC1L Monoclonal Antibody (bs-7773R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: HL60 Cells; Lane 2: NIH/3T3 Cells; Lane 3: U2OS Cells; Lane 4: HL60 Cells; Probed with SPECC1L Monoclonal Antibody (bs-7773R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: HL60 Cells; Lane 2: NIH/3T3 Cells; Lane 3: U2OS Cells; Lane 4: HL60 Cells; Probed with SPECC1L Monoclonal Antibody (bs-7773R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

SPECC1L Polyclonal Antibody

BS-7773R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSPECC1L
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Overview

  • Supplier
    Bioss
  • Product Name
    SPECC1L Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID23384
  • Target name
    SPECC1L
  • Target description
    sperm antigen with calponin homology and coiled-coil domains 1 like
  • Target synonyms
    CYTSA, GBBB2, OBLFC1, TBHS, TBHS1, cytospin-A, SPECC1-like protein, cytokinesis and spindle organization A, renal carcinoma antigen NY-REN-22
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of SPECC1L using anti-SPECC1L antibody (A09120-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPECC1L antigen affinity purified polyclonal antibody (Catalog # A09120-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPECC1L at approximately 150 kDa. The expected band size for SPECC1L is at 125 kDa.
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