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Anti-Spectrin [RG/26]

Ab01319-10.0
Absolute Antibody
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSPTB
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Spectrin [RG/26]
  • Delivery Days Customer
    7
  • Application Supplier Note
    IHC: RG/26 antibody was used to stain membranes of iRBCs infected with a parasite in a study which investigated influence of vascular endothelial growth factor on red cells infected with Plasmodium falciparum (Sachanonta et al., 2008). WB: This antibody was also used in Western blot analysis to assess free and degraded spectrin as an element of a study which aimed at investigating the infuence of constant storage temperatures and multiplewarming cycles on stored red blood cells (Wagner et al., 2014).
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; IF
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RG/26
  • Gene ID6710
  • Target name
    SPTB
  • Target description
    spectrin beta, erythrocytic
  • Target synonyms
    beta-I spectrin; EL3; HS2; HSPTB1; membrane cytoskeletal protein; Sp beta; spectrin beta chain, erythrocyte; spectrin beta chain, erythrocytic; spectrin beta Tandil; SPH2
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP11277
  • Protein Name
    Spectrin beta chain, erythrocytic
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was raised by immunising mice with red cell membranes.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of beta 1 Spectrin/SPTB using anti-beta 1 Spectrin/SPTB antibody (A02392). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-beta 1 Spectrin/SPTB antigen affinity purified polyclonal antibody (Catalog # A02392) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for beta 1 Spectrin/SPTB at approximately 270 kDa. The expected band size for beta 1 Spectrin/SPTB is at 246 kDa.
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