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Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Anti-SPR Antibody

HPA039505
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSPR
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SPR
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6697
  • Target name
    SPR
  • Target description
    sepiapterin reductase
  • Target synonyms
    SDR38C1; sepiapterin reductase; sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase); Sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming); short chain dehydrogenase/reductase family 38C, member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35270
  • Protein Name
    Sepiapterin reductase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA214577(SPR Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
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Figure 1. Western blot analysis of SPR using anti-SPR antibody (A00416-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPR antigen affinity purified polyclonal antibody (Catalog # A00416-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPR at approximately 28KD. The expected band size for SPR is at 28KD.
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