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Anti-SRY [RAB-C98]

Ab01892-1.1
Absolute Antibody
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetSRY
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-SRY [RAB-C98]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody has been used and recommended for the following methods: FC; spiked-IP;.
  • Applications
    Flow Cytometry
  • Applications Supplier
    FC; spiked-IP;
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RAB-C98
  • Gene ID6736
  • Target name
    SRY
  • Target description
    sex determining region Y
  • Target synonyms
    SRXX1, SRXY1, TDF, TDY, sex-determining region Y protein, essential protein for sex determination in human males, sex-determining region on Y, testis-determining factor on Y
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ05066
  • Protein Name
    Sex-determining region Y protein
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was obtained by recombinant antibody (rAb) phage display recognizing SRY protein under non-denaturing conditions.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SRY using anti-SRY antibody (A00614-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRY antigen affinity purified polyclonal antibody (Catalog # A00614-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRY at approximately 26KD. The expected band size for SRY is at 24KD.
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