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Western Blot analysis of HeLa cells using SRY Polyclonal Antibody
Western Blot analysis of HeLa cells using SRY Polyclonal Antibody
Western Blot analysis of HeLa cells using SRY Polyclonal Antibody

SRY Antibody

CSB-PA005928
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetSRY
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Overview

  • Supplier
    Cusabio
  • Product Name
    SRY Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6736
  • Target name
    SRY
  • Target description
    sex determining region Y
  • Target synonyms
    essential protein for sex determination in human males; sex-determining region on Y; sex-determining region Y protein; SRXX1; SRXY1; TDF; TDY; testis-determining factor on Y; truncated sex-determining region Y protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ05066
  • Protein Name
    Sex-determining region Y protein
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SRY using anti-SRY antibody (A00614-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRY antigen affinity purified polyclonal antibody (Catalog # A00614-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRY at approximately 26KD. The expected band size for SRY is at 24KD.
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ELISA analysis of antigen using GTX60628 SRY antibody [1G4]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng
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