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Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Anti-SSR3 Antibody

HPA014906
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSSR3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SSR3
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6747
  • Target name
    SSR3
  • Target description
    signal sequence receptor subunit 3
  • Target synonyms
    TRAPG, translocon-associated protein subunit gamma, SSR gamma, TRAP-complex gamma subunit, TRAP-gamma, signal sequence receptor subunit gamma, signal sequence receptor, gamma (translocon-associated protein gamma), translocon-associated protein gamma subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UNL2
  • Protein Name
    Translocon-associated protein subunit gamma
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of SSR3 using anti-SSR3 antibody (PA1796). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSR3 antigen affinity purified polyclonal antibody (Catalog # PA1796) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSR3 at approximately 21 kDa. The expected band size for SSR3 is at 21 kDa.
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