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Western blot All lanes: SSR3 antibody at 10ug/ml Lane 1: Hela whole cell lysate Lane 2: U87 whole cell lysate Lane 3: A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 22, 23 kDa Observed band size: 22 kDa
Western blot All lanes: SSR3 antibody at 10ug/ml Lane 1: Hela whole cell lysate Lane 2: U87 whole cell lysate Lane 3: A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 22, 23 kDa Observed band size: 22 kDa
Western blot All lanes: SSR3 antibody at 10ug/ml Lane 1: Hela whole cell lysate Lane 2: U87 whole cell lysate Lane 3: A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 22, 23 kDa Observed band size: 22 kDa

SSR3 Antibody

CSB-PA892358LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSSR3
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Overview

  • Supplier
    Cusabio
  • Product Name
    SSR3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6747
  • Target name
    SSR3
  • Target description
    signal sequence receptor subunit 3
  • Target synonyms
    TRAPG, translocon-associated protein subunit gamma, SSR gamma, TRAP-complex gamma subunit, TRAP-gamma, signal sequence receptor subunit gamma, signal sequence receptor, gamma (translocon-associated protein gamma), translocon-associated protein gamma subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UNL2
  • Protein Name
    Translocon-associated protein subunit gamma
  • Scientific Description
    TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SSR3 using anti-SSR3 antibody (PA1796). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSR3 antigen affinity purified polyclonal antibody (Catalog # PA1796) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSR3 at approximately 21 kDa. The expected band size for SSR3 is at 21 kDa.
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