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Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Anti-SSSCA1 Antibody

HPA064670
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetZNRD2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SSSCA1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10534
  • Target name
    ZNRD2
  • Target description
    zinc ribbon domain containing 2
  • Target synonyms
    autoantigen p27; centromeric autoantigen (27kD); p27; protein ZNRD2; sjoegren syndrome/scleroderma autoantigen 1; Sjogren syndrome/scleroderma autoantigen 1; Sjogren's syndrome/scleroderma autoantigen 1; SSSCA1; zinc ribbon domain-containing protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60232
  • Protein Name
    Sjoegren syndrome/scleroderma autoantigen 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of SSSCA1/ZNRD2 using anti-SSSCA1/ZNRD2 antibody (A14259-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat ovary tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSSCA1/ZNRD2 antigen affinity purified polyclonal antibody (Catalog # A14259-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSSCA1/ZNRD2 at approximately 23 kDa. The expected band size for SSSCA1/ZNRD2 is at 21 kDa.
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Western blot All lanes: SSSCA1 antibody at 2microg/ml Lane 1: A549 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa
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