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Western blot All lanes: SSSCA1 antibody at 2microg/ml Lane 1: A549 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa
Western blot All lanes: SSSCA1 antibody at 2microg/ml Lane 1: A549 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa
Western blot All lanes: SSSCA1 antibody at 2microg/ml Lane 1: A549 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 22 kDa Observed band size: 22 kDa

SSSCA1 Antibody

CSB-PA022722HA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetZNRD2
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Overview

  • Supplier
    Cusabio
  • Product Name
    SSSCA1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10534
  • Target name
    ZNRD2
  • Target description
    zinc ribbon domain containing 2
  • Target synonyms
    autoantigen p27; centromeric autoantigen (27kD); p27; protein ZNRD2; sjoegren syndrome/scleroderma autoantigen 1; Sjogren syndrome/scleroderma autoantigen 1; Sjogren's syndrome/scleroderma autoantigen 1; SSSCA1; zinc ribbon domain-containing protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60232
  • Protein Name
    Sjoegren syndrome/scleroderma autoantigen 1
  • Scientific Description
    Might play a role in mitosis. Antigenic molecule. Could be a centromere-associated protein. May induce anti-centromere antibodies.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SSSCA1/ZNRD2 using anti-SSSCA1/ZNRD2 antibody (A14259-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat ovary tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSSCA1/ZNRD2 antigen affinity purified polyclonal antibody (Catalog # A14259-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSSCA1/ZNRD2 at approximately 23 kDa. The expected band size for SSSCA1/ZNRD2 is at 21 kDa.
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