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Immunohistochemical staining of human tissue using anti-ST2 (human), mAb (ST33868) (Prod. No. AG-20A-0044) at 1:100 dilution. A. Immunoperoxidase staining (cytoplasmic) of formalin-fixed, paraffin-embedded human heart (100x, brown colour). B.
Immunohistochemical staining of human tissue using anti-ST2 (human), mAb (ST33868) (Prod. No. AG-20A-0044) at 1:100 dilution. A. Immunoperoxidase staining (cytoplasmic) of formalin-fixed, paraffin-embedded human heart (100x, brown colour). B.
Immunohistochemical staining of human tissue using anti-ST2 (human), mAb (ST33868) (Prod. No. AG-20A-0044) at 1:100 dilution. A. Immunoperoxidase staining (cytoplasmic) of formalin-fixed, paraffin-embedded human heart (100x, brown colour). B.

anti-ST2 (human), mAb (ST33868)

AG-20A-0044
AdipoGen Life Sciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetIL1RL1
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Overview

  • Supplier
    AdipoGen Life Sciences
  • Product Name
    anti-ST2 (human), mAb (ST33868)
  • Delivery Days Customer
    10
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ST33868
  • Concentration
    1 mg/ml
  • Gene ID9173
  • Target name
    IL1RL1
  • Target description
    interleukin 1 receptor like 1
  • Target synonyms
    DER4; FIT-1; growth stimulation-expressed; homolog of mouse growth stimulation-expressed; IL33R; interleukin 1 receptor-related protein; interleukin-1 receptor-like 1; ST2; ST2L; ST2V; T1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ01638
  • Protein Name
    Interleukin-1 receptor-like 1
  • Scientific Description
    Monoclonal Antibody. Recognizes human ST2. Isotype: Mouse IgG1kappa. Clone: ST33868. Applications: ELISA, IHC, WB. Liquid. 0.2microm-filtered solution in PBS, pH 7.4. Contains no preservatives. The ST2 gene was originally identified as a gene induced by serum or oncogene expression in fibroblasts. The gene produces a shorter soluble secreted form (ST2) and a longer, transmembrane form (ST2L) by alternative splicing. Soluble ST2 has been shown to downregulate the expression of TLR1 and TLR4. ST2L negatively regulates TLR4 signaling and induces endotoxin tolerance, and enhances Th2 responses. IL-33 is the specific ligand for ST2L. - The ST2 gene was originally identified as a gene induced by serum or oncogene expression in fibroblasts. The gene produces a shorter soluble secreted form (ST2) and a longer, transmembrane form (ST2L) by alternative splicing. Soluble ST2 has been shown to downregulate the expression of TLR1 and TLR4. ST2L negatively regulates TLR4 signaling and induces endotoxin tolerance, and enhances Th2 responses. IL-33 is the specific ligand for ST2L.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ST2/IL1RL1 using anti-ST2/IL1RL1 antibody (A00492-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ST2/IL1RL1 antigen affinity purified polyclonal antibody (Catalog # A00492-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ST2/IL1RL1 at approximately 70 kDa. The expected band size for ST2/IL1RL1 is at 63 kDa.
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Specific interaction of human ST2 (Prod. No. AG-40A-0059) with recombinant human IL-33. An indirect competitive ELISA was performed as follows; 1) coat microtiter plate wells with hST2-Fc (10microg/ml); 2) add a varying concentrations of hIL-33 with
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