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Immunohistochemical staining of human lymph node shows moderate nuclear positivity in reaction center cells.
Immunohistochemical staining of human lymph node shows moderate nuclear positivity in reaction center cells.
Immunohistochemical staining of human lymph node shows moderate nuclear positivity in reaction center cells.

Anti-STAG2 Antibody

HPA002857
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSTAG2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-STAG2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10735
  • Target name
    STAG2
  • Target description
    STAG2 cohesin complex component
  • Target synonyms
    HPE13, MKMS, NEDXCF, SA-2, SA2, SCC3B, bA517O1.1, cohesin subunit SA-2, SA2 stromalin, SCC3 homolog 2, sister chromatid cohesion 3 homolog B, stromal antigen 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N3U4
  • Protein Name
    Cohesin subunit SA-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SA2/STAG2 using anti-SA2/STAG2 antibody (A03624-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: huamn RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SA2/STAG2 antigen affinity purified polyclonal antibody (Catalog # A03624-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SA2/STAG2 at approximately 141 kDa. The expected band size for SA2/STAG2 is at 141 kDa.
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