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ProductsBack/SA2 Recombinant Antibody, Biotin Conjugated

SA2 Recombinant Antibody, Biotin Conjugated

BSM-61669R-BIOTIN
Bioss
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSTAG2
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Overview

  • Supplier
    Bioss
  • Product Name
    SA2 Recombinant Antibody, Biotin Conjugated
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Biotin
  • Gene ID10735
  • Target name
    STAG2
  • Target description
    STAG2 cohesin complex component
  • Target synonyms
    HPE13, MKMS, NEDXCF, SA-2, SA2, SCC3B, bA517O1.1, cohesin subunit SA-2, SA2 stromalin, SCC3 homolog 2, sister chromatid cohesion 3 homolog B, stromal antigen 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N3U4
  • Protein Name
    Cohesin subunit SA-2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SA2/STAG2 using anti-SA2/STAG2 antibody (A03624-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: huamn RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SA2/STAG2 antigen affinity purified polyclonal antibody (Catalog # A03624-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SA2/STAG2 at approximately 141 kDa. The expected band size for SA2/STAG2 is at 141 kDa.
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