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Anti-STAMBPL1 Antibody

144-63955
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetSTAMBPL1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-STAMBPL1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID57559
  • Target name
    STAMBPL1
  • Target description
    STAM binding protein like 1
  • Target synonyms
    ALMalpha; AMSH-FP; AMSH-like protease; AMSH-LP; associated molecule with the SH3 domain of STAM (AMSH) - Family Protein; associated molecule with the SH3 domain of STAM (AMSH) like protein; bA399O19.2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96FJ0
  • Protein Name
    AMSH-like protease
  • Scientific Description
    STAMBPL1 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AMSH-LP/STAMBPL1 using anti-AMSH-LP/STAMBPL1 antibody (A09862-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMSH-LP/STAMBPL1 antigen affinity purified polyclonal antibody (Catalog # A09862-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMSH-LP/STAMBPL1 at approximately 50 kDa. The expected band size for AMSH-LP/STAMBPL1 is at 50 kDa.
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