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Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.

Anti-STAU1 Antibody

HPA049892
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSTAU1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-STAU1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6780
  • Target name
    STAU1
  • Target description
    staufen double-stranded RNA binding protein 1
  • Target synonyms
    PPP1R150, STAU, double-stranded RNA-binding protein Staufen homolog 1, protein phosphatase 1, regulatory subunit 150, staufen, RNA binding protein, homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95793
  • Protein Name
    Double-stranded RNA-binding protein Staufen homolog 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of Staufen/STAU1 using anti-Staufen/STAU1 antibody (PB9895). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Staufen/STAU1 antigen affinity purified polyclonal antibody (Catalog # PB9895) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Staufen/STAU1 at approximately 55 kDa. The expected band size for Staufen/STAU1 is at 63 kDa.
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