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SW480 cell lysates probed with Staufen Polyclonal Antibody, Unconjugated (bs-9877R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
SW480 cell lysates probed with Staufen Polyclonal Antibody, Unconjugated (bs-9877R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
SW480 cell lysates probed with Staufen Polyclonal Antibody, Unconjugated (bs-9877R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Staufen Polyclonal Antibody

BS-9877R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSTAU1
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Overview

  • Supplier
    Bioss
  • Product Name
    Staufen Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6780
  • Target name
    STAU1
  • Target description
    staufen double-stranded RNA binding protein 1
  • Target synonyms
    PPP1R150, STAU, double-stranded RNA-binding protein Staufen homolog 1, protein phosphatase 1, regulatory subunit 150, staufen, RNA binding protein, homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95793
  • Protein Name
    Double-stranded RNA-binding protein Staufen homolog 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • de Morrée A, van Velthoven CTJ, Gan Q, et al. Staufen1 inhibits MyoD translation to actively maintain muscle stem cell quiescence. Proc Natl Acad Sci U S A. 2017,114(43):E8996-E9005. doi: 10.1073/pnas.1708725114
    Read this paper

Datasheet

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Figure 1. Western blot analysis of Staufen/STAU1 using anti-Staufen/STAU1 antibody (PB9895). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human CACO-2 whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Staufen/STAU1 antigen affinity purified polyclonal antibody (Catalog # PB9895) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Staufen/STAU1 at approximately 55 kDa. The expected band size for Staufen/STAU1 is at 63 kDa.
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